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dna smart ladder  (Kaneka Corp)

 
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    Kaneka Corp dna smart ladder
    Dna Smart Ladder, supplied by Kaneka Corp, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/dna smart ladder/product/Kaneka Corp
    Average 90 stars, based on 1 article reviews
    dna smart ladder - by Bioz Stars, 2026-02
    90/100 stars

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    Gel-shift analysis of T. neapolitana and E. coli DNA operator regions binding to cell-free synthesized T. neapolitana ArgR and T. maritima putative regulatory proteins. Lane 1—without protein; lanes 2–5 contain 12, 24, 48, and 96 nM operator DNA. His-tagged purified proteins were probed to labeled operator DNA at 37 °C for 30 min and subjected to electrophoresis on a 2% agarose gel. ArgR binding was carried out in the presence of L-arginine in both binding and running buffers.

    Journal: BioTech

    Article Title: Cell-Free Protein Synthesis by Diversifying Bacterial Transcription Machinery

    doi: 10.3390/biotech10040024

    Figure Lengend Snippet: Gel-shift analysis of T. neapolitana and E. coli DNA operator regions binding to cell-free synthesized T. neapolitana ArgR and T. maritima putative regulatory proteins. Lane 1—without protein; lanes 2–5 contain 12, 24, 48, and 96 nM operator DNA. His-tagged purified proteins were probed to labeled operator DNA at 37 °C for 30 min and subjected to electrophoresis on a 2% agarose gel. ArgR binding was carried out in the presence of L-arginine in both binding and running buffers.

    Article Snippet: DNA concentration was determined by spectrophotometry and confirmed by comparing band intensity with a Smart Ladder reference DNA (Eurogentec, Liege, Belgium) after electrophoresis.

    Techniques: Electrophoretic Mobility Shift Assay, Binding Assay, Synthesized, Purification, Labeling, Electrophoresis, Agarose Gel Electrophoresis